#Capto butyl software#

We enable science by offering product choice, services, process excellence and our people make it happen.

#Capto butyl software#

The Assist software supports experimental set-up, data management and evaluation to enhance process understanding. Learn more about hydrophobic interaction, hydrophobic interaction chromatography. The authors declare no conflict of interest. The obtained rhIL-11 had a compact and highly ordered structure and was biologically active. PreDictor 96-well plates used in conjunction with the latest version of Assist software, help increase process development efficiency by reducing the time to develop and evaluate new processes. A two-step chromatography, utilizing Capto Butyl-S combined with Capto Q, was established to efficiently purify tag-free rhIL-11 from the supernatant of the cell lysate. Capto HIC media are available in a variety of formats for screening to large-scale manufacture, including PreDictor plates prepacked HiScreen columns for scale-up and bulk packs from 25 ml to litres for pilot studies and manufacture. These Capto media have a high tolerance to sodium hydroxide, ensuring repeated cleaning-in-place (CIP) procedures to remove tightly bound impurities and maintain capacity. At the same time, GE Healthcare has extended its range of pre-filled PreDictor 96-well plates to include eight HIC media, available as single medium plates or screening plates, facilitating high-throughput process development.Д¬apto HIC media are based on the Capto platform's rigid, high-flow agarose base matrix which enables the use of very high flow rates and larger sample volume processing, leading to higher throughput and improved process economy. Capto Phenyl and Capto Butyl are intended for the capture and intermediate stages of protein puri?cation. This new MMC resin will be useful in future mAb manufacturing platforms comprising of a robust and cost-effective flow-through polishing step.Capto Phenyl (high sub) and Capto Butyl media extend GE Healthcare's hydrophobic interaction chromatography (HIC) portfolio to offer a wide range of hydrophobicity for screening and purification. Capto Butyl, 25 mL Useful for the intermediate stages of protein purification, Capto Butyl HIC resin combines high capacity, high flow rate, and low back pressure to reduce process cycle times. In addition, the MMC flow-through polishing step could be directly coupled with a specified CEX chromatography step to efficiently remove mAb aggregates from 2.3% to 93%) with a high loading capacity around 1000В mg/mL-resin. The study revealed that the MMC resin was effective for developing one-step flow-through polishing workflow for mAb purification. Extended purification studies of mAb using the MMC resin in the flow-through polishing step were carried out under optimized pH and conductivity condition as determined in a DOE study. OPUS В® 2.5, 5 and 8 Pre-packed Chromatography Columns enable process validation and small-scale GMP manufacturing with column volumes ranging from 25 mL - 1.5 L. The optimized ligand structure displayed a wide operational conductivity range. Introducing OPUS В® 2.5 Pre-packed Column. On the other hand, modification with butyl group kept mAb yield more than 95%. That is 25% increase of phenyl group modification ratio reduces mAb yield from 95% to 90%. We also found that structure of hydrophobic groups in the ligand mainly influenced on mAb yield. The resulting hydrophobic modified PAA ligand reduced HCP level to 12% (21вЂ“23В ppm) under 6 mS/cm in a flow-through polishing step of mAb, while not modified PAA ligand showed only 79% (145В ppm). Excellent for method optimization and parameter screening due to the 10 cm bed height. We have developed novel MMC resins based on PAA polymer ligand partially modified with hydrophobic butyl or phenyl group. Capto Phenyl (High sub) and Capto Butyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification. Polyallylamine (PAA) has been utilized as a salt tolerant anion exchange chromatography ligand in downstream processing of biopharmaceuticals.